Finds annotations for DNA sequence in a local BLAST database.
BLAST+ is a newer version of the BLAST package and is recommended to use by the NCBI.
BLAST+ is used as an external tool from UGENE and it must be installed on your system. To learn more about the external tools, please, read main UGENE User Manual.
Parameters in GUI
| Parameter | Description | Default value |
|---|---|---|
| Database name (required) | Base name for BLAST DB files. | |
| Database path (required) | Path to the database files. | |
| Tool path (required) | Path to a BLAST+ executable. | default |
| Search type (required) | Selects the type of BLAST searches. | blastn |
| Annotate as | Name of the result annotations. | misc_feature |
| Expected value | Expectation threshold value. | 10 |
| Temporary directory (required) | Directory for temporary files. | default |
Parameters in Workflow File
Type: blast-plus
| Parameter | Parameter in the GUI | Type |
|---|---|---|
| db-name | Database name | string |
| db-path | Database path | string |
| tool-path | Tool path | string |
| blast-type | Search type | string Available values are:
|
| result-name | Annotate as | string |
| e-val | Expected value | numeric |
| temp-dir | Temporary directory | string |
Input/Output Ports
The element has 1 input port:
Name in GUI: Input sequence
Name in Workflow File: in-sequence
Slots:
| Slot In GUI | Slot in Workflow File | Type |
|---|---|---|
| Sequence | sequence | sequence |
And 1 output port:
Name in GUI: Annotations
Name in Workflow File: out-annotations
Slots:
| Slot In GUI | Slot in Workflow File | Type |
|---|---|---|
| Set of annotations | annotations | annotation-table |