The Primer3 plugin is a port of the Primer3 tool. It is intended to pick primers from a DNA sequence.
To use the Primer3, open a DNA sequence and select the Analyze ‣ Primer3 context menu item. The dialog will appear:
All available parameters are the same as in the original Primer3.
However there is one additional feature available which is not originally a part of Primer3 tool. It allows user design primers for RT-PCR experiments by choosing which exons/introns to span with the primer product. This feature is described in detailed below.