Primers for the running In Silico PCR can be chosen from a primers library. Primers library it is a storage for keeping user primers. The added primers are stored between UGENE sessions.
To choose a primer from the primers library click on the following button:
The following dialog will appear:
The table consists of the following columns: name, GC-content (%), Tm, Length (bp) and sequence. Select primer in the table and click on the Choose button.
To configure primers library go to the Tools->Primer->Primer library context menu. The following window will appear:
- The table columns are: name, …
- Click “New Primer” for adding a new one.
- Select primers in the table (you can use Ctrl and Shift) and then click “Remove” for removing the selected primers from the library.