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You can select the entire sequence, a custom region, or a selected region if there was one. The region must be less than 3000 bases. It is acceptable to select a small region (<10bp), but there will almost certainly be no folding there.
Rules for substituting the selected region:
If several regions have been selected in a sequence (using the GenBank format), then the dialog will only accept the first of them. The remaining selected regions are ignored.
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But there is an exception: if the sequence is marked circular and the region passing through the end/beginning is selected, then such a region is considered as a whole region and mfold will be launched on it as on an ordinary piece of the sequence (2 parts of the region – one that goes to end and another that starts from the beginning of the sequence – will be combined into one sequence and analyzed). This is the only case where the start of a region can be greater than the end:
Output settings
UGENE&Ghostscript settings in the "Output" tab.
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