Workflow Designer Documentation v. 1.16.0
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  • Raw RNA-Seq Processing

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changes.mady.by.user Olga Golosova

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changes.mady.by.user Yuliya Algaer

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HTML
<center>
  <br>
  <img src="/wiki/download/attachments/13008932/raw_rnaseq_single-end_with_tophat14058846/Raw RNA-Seq Processing.png"/>
  <br> 
</center>

The workflow with mapping for paired-end short appearance is the following:

HTML
<center>
  <br>
  <img src="/wiki/download/attachments/14058846/Raw RNA-Seq Processing_1.png"/>
  <br> 
</center>
Workflow Wizard

The wizard for single-end reads has 5 page.

  1. Input data: On this page you must input FASTQ file(s). 

    HTML
    <center>
  <br>
  <img src="/wiki/download/attachments/14058846/Raw RNA-Seq Processing_2.png"/>
  <br> 
</center>

  2. Pre-processing: On this page you can modify filtration parameters. 

    HTML
    <center>
  <br>
  <img src="/wiki/download/attachments/14058846/Raw RNA-Seq Processing_3.png"/>
  <br> 
</center>

  3. Mapping: On this page you must input reference and optionally modify advanced parameters. 

    HTML
    <center>
  <br>
  <img src="/wiki/download/attachments/

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  1. 14058846/Raw RNA-Seq Processing_4.png"/>
  <br> 
</center>

  2. Output data: On this page you must input output parameters. 

    HTML
    <center>
  <br>
  <img src="/wiki/download/attachments/14058846/Raw RNA-Seq Processing_5.png"/>
  <br> 
</center>

The wizard for paired-end reads has 5 page.

  1. Input data: On this page you must input FASTQ file(s). 

    HTML
    <center>
  <br>
  <img src="/wiki/download/attachments/14058846/Raw RNA-Seq Processing_6.png"/>
  <br> 
</center>

  2. Pre-processing: On this page you can modify filtration parameters. 

    HTML
    <center>
  <br>
  <img src="/wiki/download/attachments/14058846/Raw RNA-Seq Processing_7.png"/>
  <br> 
</center>

  3. Mapping: On this page you must input reference and optionally modify advanced parameters. 

    HTML
    <center>
  <br>
  <img src="/wiki/download/attachments/14058846/Raw RNA-Seq Processing_8.png"/>
  <br> 
</center>

  4. Output data: On this page you must input output parameters. 

    HTML
    <center>
  <br>
  <img src="/wiki/download/attachments/14058846/Raw RNA-Seq Processing_9.png"/>
  <br> 
</center>