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- A - the pair of primers closest to the amplified fragment, corresponding to the entire set of parameters.
- B1 - the
- the pair of primers closest to the amplified fragment, corresponding to the entire set of parameters ,
- if B2 and/or B3 exist. Could be the same as A, but not always.
- B2 - the pair of primers corresponding to the entire set of parameters, which has a 4-nucleotide intersection with B1.
- B3 - the
- the pair of primers corresponding to the entire set of parameters, which starts immediately when B1 ends.
Also, you may see results in the result table. Single A single click on the result in the result table select selects the corresponding annotation, double click - export the result resulting primer to the separate file (with the backbone sequence, which will be added during the exporting process).
User primer
You may check the validity of the pair of primers by typing this pair into the corresponding fields. Also, you may add your primers 5' or/and 3' 8 bases endings, which do not form simple unwanted connections.