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This tab contains actions, which could be processed for found primers.

Check complementary

The "Check complementary" subprocess is responsible for result primers filtering. If primer pair has selfdimers or heterodimer with dimer length/dimer GC-content more or equal, than was set, this primer will be filtered and won't be considered as a result of Primer3 calculation.

If "Check complementary" has been set, the result report will contain the additional table, which shows, what primers have been filtered and why:

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